Haematologica
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Haematologica, Vol 82, Issue 3, 334-335
Copyright © 1997 by Ferrata Storti Foundation

Comparative Study

Differences in phycoerythrin- or fluorescein-isothiocyanate conjugated 8G12 on CD34+ cell evaluation

F Ortuno, F Ferrer, ML Lozano, I Heras, JM Moraleda, and V Vicente

Unit of Hematology and Hemotherapy, School of Medicine, Hospital General Universitario, Murcia, Spain.

To evaluate the equivalence of 8G12 conjugated with either phycoerythrin (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Significantly higher values (p < 0.001) were observed in PE-CD34+ cells when compared with FITC-CD34+ cells both in duplicate and simultaneous analysis. Our data suggest that the choice of fluorochrome is relevant in the measurement of CD34+ cells with 8G12.




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Copyright © 1997 by the Ferrata Storti Foundation.